(KO Validated) Bcl-2 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90319A
Western blot analysis of extracts of various cell lines using Bcl-2 Polyclonal Antibody at 1:500 dilution.
Western blot analysis of extracts from wild type(WT) and Bcl-2 knockout (KO) 293T cells using Bcl-2 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human thyroid cancer using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using Bcl-2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using Bcl-2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Product Name
(KO Validated) Bcl-2 Polyclonal Antibody
Catalog Number
RD90319A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
Immunogen Information
Immunogen
A synthetic peptide of human Bcl-2
Gene ID
596
Swissprot
P10415
Synonyms
BCL2Bcl-2PPP1R50apoptosis regulator Bcl-2
Calculated MW
58kDa/59kDa/60kDa
Observed MW
Refer to figures
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas