(KO Validated) DNMT1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD83344A
Western blot analysis of extracts of various cell lines using DNMT1 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts from normal (control) and DNMT1 knockout (KO) 293T cells using DNMT1 Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of 293T cells using DNMT1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Product Name
(KO Validated) DNMT1 Polyclonal Antibody
Catalog Number
RD83344A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.
Immunogen Information
Immunogen
Recombinant fusion protein of human DNMT1 (NP_001370.1).
Gene ID
1786
Swissprot
P26358
Synonyms
ADCADNAIMCXXC9DNMTHSN1EMCMTm.HsaIDNMT1
Calculated MW
144 kDa/183 kDa/184 kDa
Observed MW
200 kDa
Applications
Reactivity
Human
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas