(KO Validated) PARP1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90291A
Western blot analysis of extracts from wild type(WT) and PARP1 knockout (KO) HeLa cells, using PARP1 Polyclonal Antibody at 1:5000 dilution.
![Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] PARP1 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](/upload/ec/antibodies/RD90291A-IHC01.jpg)
Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] PARP1 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
![Immunofluorescence analysis of C6 cells using [KO Validated] PARP1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](/upload/ec/antibodies/RD90291A-IF01.jpg)
Immunofluorescence analysis of C6 cells using [KO Validated] PARP1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
![Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] PARP1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](/upload/ec/antibodies/RD90291A-IF02.jpg)
Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] PARP1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
![Immunofluorescence analysis of U2OS cells using [KO Validated] PARP1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](/upload/ec/antibodies/RD90291A-IF03.jpg)
Immunofluorescence analysis of U2OS cells using [KO Validated] PARP1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Product Name
(KO Validated) PARP1 Polyclonal Antibody
Catalog Number
RD90291A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
Immunogen Information
Immunogen
A synthetic peptide of human PARP1
Gene ID
142
Swissprot
P09874
Synonyms
PARP1ADPRTADPRT 1ADPRT1ARTD1PARPPARP-1PPOLpADPRT-1poly(ADP-ribose) polymerase 1
Calculated MW
97kDa
Observed MW
43KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:100,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.