CDK4 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD252141A
Western blot analysis of Hela cell lysate using CDK4 Polyclonal Antibody at dilution of 1:1000
Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CDK4 Polyclonal Antibody at dilution of 1:60(×200)
Product Name
CDK4 Polyclonal Antibody
Catalog Number
RD252141A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.
Immunogen Information
Immunogen
Fusion protein of human CDK4
Swissprot
P11802
Synonyms
Cdk 4cdk4CDK4 proteinCDK4Cell division kinase 4Cell division protein kinase 4CMM 3CMM3Crk3Cyclin dependent kinase 4Cyclin-dependent kinase 4Melanoma cutaneous malignant 3MGC14458p34 cdk4PSK J3PSK-J3
Calculated MW
34 kDa
Observed MW
Refer to figures
Gene Accession
BC003644
Applications
Reactivity
Human, Mouse, Rat
Tested Applications
WB,IHC,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:50-1:300, ELISA 1:5000-1:10000
Concentration
1.02 mg/mL
Storage Buffer
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas