DNMT1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90142A
Western blot analysis of extracts of various cell lines using DNMT1 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using DNMT1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat spleen using DNMT1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human tonsil using DNMT1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human lung cancer using DNMT1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug DNMT1 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using DNMT1 Polyclonal Antibody at a dilution of 1:1000.
Product Name
DNMT1 Polyclonal Antibody
Catalog Number
RD90142A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.
Immunogen Information
Immunogen
Recombinant fusion protein of human DNMT1
Gene ID
1786
Swissprot
P26358
Synonyms
ADCADNAIMCXXC9DNMTHSN1EMCMTm.HsaIDNMT1
Calculated MW
31kDa/49kDa
Observed MW
55KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IP 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas