EIF2 alpha Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD71102A
Western Blot analysis of various cells using EIF2 alpha Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using EIF2 alpha Polyclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of Rat kidney tissue using EIF2 alpha Polyclonal Antibody at dilution of 1:200.
Product Name
EIF2 alpha Polyclonal Antibody
Catalog Number
RD71102A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins.
Immunogen Information
Immunogen
Synthesized peptide derived from human eIF2α around the non-phosphorylation site of Ser51.
Swissprot
P05198
Synonyms
EIF 2 alphaEIF 2EIF 2AEIF 2alphaeIF-2-alphaeIF-2AEIF-2alphaEIF2 alphaEIF2EIF2AEIF2S1Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDaEukaryotic translation initiation factor 2 subunit 1 alphaEukaryotic translation initiation fa
Calculated MW
36 kDa
Observed MW
38 kDa
Applications
Reactivity
Human,Mouse,Rat,Monkey
Tested Applications
WB,IHC-p,IF,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200, ELISA 1:10000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas