For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90350A
Western blot analysis of extracts of various cell lines using FSP1/S100A4 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human colon using FSP1/S100A4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human liver using FSP1/S100A4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Immunogen Information
Immunogen
Recombinant fusion protein of human FSP1/S100A4
Gene ID
6275
Swissprot
P26447
Synonyms
S100A418A242ACAPLFSP1MTS1P9KAPEL98protein S100-A4
Calculated MW
48kDa/50kDa/58kDa/60kDa/63kDa
Observed MW
58-68KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:100
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.