GARS Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD83301A
Western blot analysis of extracts of various cell lines using GARS Polyclonal Antibody at dilution of 1:5000.
Immunohistochemistry of paraffin-embedded Rat brain using GARS Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse brain using GARS Polyclonal Antibody at dilution of 1:100 (40x lens).
Confocal immunofluorescence analysis of NIH-3T3 cells using GARS Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using GARS Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Product Name
GARS Polyclonal Antibody
Catalog Number
RD83301A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Two transcript variants encoding different isoforms have been found for this gene.
Immunogen Information
Immunogen
Recombinant fusion protein of human GARS (NP_002038.2).
Gene ID
2617
Swissprot
P41250
Synonyms
GARSCMT2DDSMAVGlyRSHMN5SMAD1
Calculated MW
83 kDa
Observed MW
83 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:1000-1:4000 IHC 1:50-1:200 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas