GFP Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86712A
Western blot of eGFP protein with anti-eGFP at dilution of 1:80000.
Immunohistochemical analysis of transgenic zebrafish embryo with anti-GFP antibody at dilution of 1:800.
Immunofluorescent analysis of 293F cells transfected with the FLAG-GFP, using anti-GFP-Tag Monoclonal Antibody at dilution of 1:8000.
IP Result of 293 cells transfected with GFP-Tag fusion protein,using anti-GFP-Tag mouse monoclonal antibody. Lane 1:input, lane 2: mouse IgG2a Isotype Control, Lane 3: anti-GFP-Tag monoclonal antibody
1x10^6 CHO cells Transfected with a GFP plasmid were stained with 0.2ug Anti-GFP tag mAb(RD86712A) and AF647 conjugated Goat Anti-mouse IgG (H+L);lsotype Control stained with 0.2ug mouse IgG and AF647 conjugated Goat Anti-mouse IgG (H+L)
Product Name
GFP Monoclonal Antibody
Catalog Number
RD86712A
Clonality
Monoclonal
Purification Method
Protein A/G purification
Isotype
IgG2a
Host
Mouse
Background
The green fluorescent protein (GFP) is a protein that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. It is first isolated from the jellyfish Aequorea victoria and is frequently used as a reporter of expression. GFP is widely used in gene expression, tracing and protein subcellualr localization.
Immunogen Information
Immunogen
Recombinant GFP protein expressed by E.coli
Swissprot
P42212
Synonyms
GFPGreen fluorescent protein
Calculated MW
27kDa
Observed MW
27kDa
Applications
Reactivity
All
Tested Applications
WB, IF,IP,FCM
Conjugation
Unconjugated
Dilution
WB 1:100000-1:200000, IP 3ug/sample, IF 1:8000-1:16000,FCM:0.2 ug
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.