Histone H1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD71282A
Western Blot analysis of 3T3 cells using Histone H1 Polyclonal Antibody at dilution of 1:1000.
Product Name
Histone H1 Polyclonal Antibody
Catalog Number
RD71282A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
May play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. Essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling.
Immunogen Information
Immunogen
Synthesized peptide derived from human Histone H1 around the non-phosphorylation site of Thr17.
Swissprot
P16401,P16402,P10412
Synonyms
HIST1H1BH1F5Histone H1.5Histone H1aHistone H1bHistone H1s-3HIST1H1DH1F3Histone H1.3Histone H1cHistone H1s-2HIST1H1EH1F4Histone H1.4Histone H1bHistone H1s-4
Calculated MW
23 kDa
Observed MW
31
28 kDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB,IHC-p,IF,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:20000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas