For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86816A
Western blot analysis of extracts of various cell lines using MRP4/ABCC4 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat testis using MRP4/ABCC4 Polyclonal Antibody at dilution of 1:150 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse testis using MRP4/ABCC4 Polyclonal antibody at dilution of 1:150 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human oophoroma using [KO Validated] MRP4/ABCC4 Polyclonal antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human prostate cancer using [KO Validated] MRP4/ABCC4 Polyclonal antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This family member plays a role in cellular detoxification as a pump for its substrate, organic anions. It may also function in prostaglandin-mediated cAMP signaling in ciliogenesis. Alternative splicing of this gene results in multiple transcript variants.
Immunogen Information
Immunogen
Recombinant fusion protein of human MRP4 / ABCC4
Gene ID
10257
Swissprot
O15439
Synonyms
ABCC4MOAT-BMOATBMRP4
Calculated MW
30kDa/96kDa
Observed MW
105kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.