Nestin Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD80727A
Western blot analysis of extracts of various cell lines using Nestin Polyclonal Antibody at dilution of 1:3000.
Immunohistochemistry of paraffin-embedded Mouse brain using Nestin Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-251MG cells using Nestin Polyclonal Antibody at dilution of 1:100 (20x lens). Blue: DAPI for nuclear staining.
Product Name
Nestin Polyclonal Antibody
Catalog Number
RD80727A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells.
Immunogen Information
Immunogen
Recombinant fusion protein of human Nestin (NP_006608.1).
Gene ID
10763
Swissprot
P48681
Synonyms
NESNbla00170nestinNestin
Calculated MW
177 kDa
Observed MW
180 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000 IHC 1:50-1:100 IF 1:50-1:100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.