PCNA Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86715A
Western blot of Zebrafish whole lysates with anti-PCNA monoclonal antibody at dilution of 1:1000.
Immunofluorescence of Zebrafish frozen section with anti-PCNA monoclonal antibodyat dilution of 1:100.
Immunohistochemistry of Zebrafish paraffin embedded section with anti-PCNA mcab at dilution of 1:100.
Product Name
PCNA Monoclonal Antibody
Catalog Number
RD86715A
Clonality
Monoclonal
Purification Method
Protein A/G purification
Isotype
IgG1
Host
Mouse
Background
Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics. PCNA gene is often stably and constitutively expressed in most tissues and cells, it is commonly used as a loading control for western blot and other test.
Immunogen Information
Immunogen
Recombinant human PCNA protein expressed by E.coli
Gene ID
5111
Swissprot
P12004
Synonyms
ATLD2cb16CyclinDNA polymerase delta auxiliary proteinetID36690.10fa28e03fb36g03HGCN8729MGC8367Mutagen-sensitive 209 proteinOTTHUMP00000030189OTTHUMP00000030190Pcna/cyclinPCNARPolymerase delta accessory proteinProliferating ce
Calculated MW
36 kDa
Observed MW
36 kDa
Applications
Reactivity
Zebrafish
Tested Applications
WB,IF,IHC
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000
Concentration
2mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.