PDPK1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD71631A
Western Blot analysis of COLO205 cells using PDPK1 Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of Rat lung tissue using PDPK1 Polyclonal Antibody at dilution of 1:200.
Product Name
PDPK1 Polyclonal Antibody
Catalog Number
RD71631A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene.
Immunogen Information
Immunogen
Synthesized peptide derived from human PDK1 around the non-phosphorylation site of Ser241.
Swissprot
O15530
Synonyms
3 phosphoinositide dependent protein kinase 13-phosphoinositide-dependent protein kinase 1hPDK 1hPDK1MGC20087MGC35290OTTHUMP00000159109OTTHUMP00000159110OTTHUMP00000174525PDK1Pdpk1PDPK1PDPK2PDPK2PPkB kinasePkB kinase like gene 1PkB like 1
Calculated MW
63 kDa
Observed MW
60 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:10000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas