Recombinant 4E BP1 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86993A
Western blot detection of eIF4EBP1 in Jurkat,C6,CHO-K1 cell lysates using eIF4EBP1 Rabbit mAb(1:1000 diluted).Predicted band size:13kDa.Observed band size:15-20kDa.
Immunohistochemistry of eIF4EBP1 in paraffin-embedded Human breast cancer tissue using eIF4EBP1 Rabbit mAb at dilution 1:50
Product Name
Recombinant 4E BP1 Monoclonal Antibody
Catalog Number
RD86993A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Eukaryotic translation initiation factor 4E-binding protein 1(4EBP1),is a member of 4EBPs family,which regulate the translation of a subset of mRNA by competing with eIF4G for binding to eIF4E,thus preventing the assembly of the eIF4F complex. The eIF4F facilitates the recruitment of other translation initiation factors to form the complex and then initiates cap-dependent translation.4EBP1 also mediates the regulation of protein translation by growth factors,hormones and other stimuli that signal through the MAP kinase and mTORC1 pathways. There are three forms of 4EBP1,alpha,beta and gamma.
Immunogen Information
Immunogen
A synthetic peptide of human eIF4EBP1
Gene ID
1978
Swissprot
Q13541
Synonyms
4E-BP14EBP14EBP1BP 1eIF4E binding protein 1eIF4E-binding protein 1Eif4ebp1Eukaryotic translation initiation factor 4E-binding protein 1PHAS-IPHASIPhosphorylated heat- and acid-stable protein regulated by insulin 1
Calculated MW
67kDa
Observed MW
67kDa
Applications
Reactivity
Human,Mouse,Rat,Hamster
Tested Applications
WB,IHC-P
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas