Recombinant Beclin 1 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86920A
Western blot detection of Beclin 1 in K562,Rat Brain,C6,3T3,Hela cell lysates using Beclin 1 Rabbit mAb(1:1000 diluted).Predicted band size:52kDa.Observed band size:60kDa.
Product Name
Recombinant Beclin 1 Monoclonal Antibody
Catalog Number
RD86920A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Beclin-1 (BECN1), also known as ATG6 or VPS30, has a central role in autophagy, a process of programmed cell survival, which is increased during periods of cell stress and extinguished during the cell cycle. Beclin-1, may play a role in antiviral host defense. It protects against infection by a neurovirulent strain of Sindbis virus. Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration. A progressively reduced Beclin-1 expression is reported to correlate with the primary tumor growth of squamous cell carcinoma and adenocarcinoma of the lung. Caspase-mediated cleavage of Beclin 1 promotes crosstalk between apoptosis and autophagy.
Immunogen Information
Immunogen
Recombinant protein of human Beclin 1
Gene ID
8678
Swissprot
Q14457
Synonyms
APG6ATG 6ATG6ATG6 autophagy related 6 homologBcl-2-interacting protein beclinBeclin 1 (coiled coil moesin like BCL2 interacting protein)Beclin 1 autophagy relatedBeclin-1Beclin1BECN 1Becn1BECN1Coiled coil myosin like BCL2 interacting proteinC
Calculated MW
48/53 kDa
Observed MW
46/54kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.