Recombinant ENO1 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86927A
Western blot detection of ENO1 in Jurkat,Rat Brain,C6,Hela cell lysates using ENO1 Rabbit mAb(1:1000 diluted).Predicted band size:47kDa.Observed band size:47kDa.
Immunohistochemistry of ENO1 in paraffin-embedded Human tonsil using ENO1 Rabbit mAb at dilution 1:100
Immunofluorescence of ENO1(green) in Hela cells using ENO1 Rabbit mAb at dilution 1:50, and DAPI(blue)
Product Name
Recombinant ENO1 Monoclonal Antibody
Catalog Number
RD86927A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate.Enolase were down-regulated in oxLDL-treated cells or in VLDL-treated cells .And it identified which are associated with glucose metabolism were down-regulated in the process of foam cells formation.
Immunogen Information
Immunogen
A synthetic peptide of human ENO1
Gene ID
2023
Swissprot
P06733
Synonyms
2 phospho D glycerate hydro lyase2-phospho-D-glycerate hydro-lyaseAlpha enolaseAlpha enolase like 1Alpha-enolaseC myc promoter binding proteinC-myc promoter-binding proteinEC 4.2.1.11eno1ENO1L1ENOAEnolase 1 (alpha)Enolase 1 (alpha) like 1Enol
Calculated MW
56kDa
Observed MW
56kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-P,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:100-1:200, IF 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.