Recombinant Nampt Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86988A
Western blot detection of Visfatin in Hela,A549,HL-60,U2OS,C6 cell lysates using Visfatin Rabbit mAb(1:1000 diluted).Predicted band size:56kDa.Observed band size:56kDa.
Immunohistochemistry of Visfatin in paraffin-embedded Human lung cancer tissue using Visfatin Rabbit mAb at dilution 1:50
Product Name
Recombinant Nampt Monoclonal Antibody
Catalog Number
RD86988A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Nicotinamide phosphoribosyltransferase(NAMPT) has two usual synonyms termed Visfatin and PBEF. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide,an intermediate in the biosynthesis of NAD,which is the rate limiting component in the mammalian NAD biosynthesis pathway. NAMPT is localized in cytoplasm and expressed in large amounts in bone marrow,liver tissue,and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. NAMPT levels are altered in plasma of patients with type 2 diabetes mellitus (T2DM),and it is now evidenced that NAMPT may plays a role in lipid metabolism.
Immunogen Information
Immunogen
A synthetic peptide of human Visfatin
Gene ID
10135
Swissprot
P43490
Synonyms
1110035O14RikAI314458AI480535DKFZP666B131EC 2.4.2.12MGC117256NAmPRTaseNamptNAMPTNicotinamide phosphoribosyltransferasePBEF 1PBEFPBEF1Pre B cell colony enhancing factor 1Pre B cell colony enhancing factorPre B cell enhancing factorPre-B cel
Calculated MW
160kDa
Observed MW
160kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-P
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.