Recombinant Nrf1 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87061A
Western blot detection of NRF1 in C6,3T3,Hela cell lysates using NRF1 Rabbit mAb(1:1000 diluted).Predicted band size:54kDa.Observed band size:68kDa.
Immunohistochemistry of NRF1 in paraffin-embedded Human colon cancer tissue using NRF1 Rabbit mAb at dilution 1:50
Immunofluorescence of NRF1(green) in Hela cells using NRF1 Rabbit mAb at dilution 1:200, and DAPI(blue)
Product Name
Recombinant Nrf1 Monoclonal Antibody
Catalog Number
RD87061A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants.
Immunogen Information
Immunogen
A synthetic peptide of human NRF1
Gene ID
4899
Swissprot
Q16656
Synonyms
alpha palalpha palindromic binding proteinAlpha palindromic-binding proteinAlpha-pallocus control region factor 1NFE2 related factor 1NRF-1Nrf1NRF1Nuclear respiratory factor 1
Calculated MW
142kDa
Observed MW
130/180kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-P,IF
Conjugation
Unconjugated
Dilution
WB 1:2000, IHC 1:50-1:100, IF 1:50-1:200
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas