Recombinant PP1C beta Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87071A
Western blot detection of PPP1CB in K562,Rat Brain,C6,3T3,Hela cell lysates using PPP1CB Rabbit mAb(1:1000 diluted).Predicted band size:37kDa.Observed band size:37kDa.
Immunohistochemistry of PPP1CB in paraffin-embedded Human colon cancer tissue using PPP1CB Rabbit mAb at dilution 1:100
Immunofluorescence of PPP1CB(green) in Hela cells using PPP1CB Rabbit mAb at dilution 1:50, and DAPI(blue)
Product Name
Recombinant PP1C beta Monoclonal Antibody
Catalog Number
RD87071A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed.
Immunogen Information
Immunogen
A synthetic peptide of human PPP1CB
Gene ID
5500
Swissprot
P62140
Synonyms
MGC3672PP 1BPP-1BPP1BPP1BPP1betaPPP1CBPPP1CDProtein phosphatase 1 betaProtein phosphatase 1 catalytic subunit beta isoformProtein phosphatase 1 deltaProtein phosphatase 1catalytic subunitbeta isozymeProtein phosphatase 1catalytic subunitde
Calculated MW
53kDa
Observed MW
20kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-P,IF
Conjugation
Unconjugated
Dilution
WB 1:1000-1:3000, IHC 1:50-1:100, IF 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas