Recombinant Smad3 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86910A
Western blot detection of Smad3 in Jurkat,C6,Hela cell lysates using Smad3 Rabbit mAb(1:1000 diluted).Predicted band size:48kDa.Observed band size:52kDa.
Immunohistochemistry of Smad3 in paraffin-embedded Human breast cancer tissue using Smad3 Rabbit mAb at dilution 1:100
Product Name
Recombinant Smad3 Monoclonal Antibody
Catalog Number
RD86910A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
Immunogen Information
Immunogen
A synthetic peptide of human Smad3
Gene ID
4088
Swissprot
P84022
Synonyms
DKFZP586N0721DKFZp686J10186hMAD 3hMAD-3hSMAD3HSPC193HST17436JV15 2JV15-2JV152LDS1CLDS3MAD (mothers against decapentaplegic Drosophila) homolog 3MAD homolog 3Mad homolog JV15 2Mad protein homologMADmothers against decapentaplegic homolog 3
Calculated MW
54kDa
Observed MW
54kDa
Applications
Reactivity
Human,Rat
Tested Applications
WB,IHC-P
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000, IHC 1:20-1:500
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.