RPL23A Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD83020A
Western blot analysis of extracts of various cell lines using RPL23A Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using RPL23A Polyclonal Antibody at dilution of 1:1000.
Confocal immunofluorescence analysis of HeLa cells using RPL23A Polyclonal Antibody at dilution of  1:100. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of HeLa cells using RPL23A Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of A-431 cells using RPL23A Polyclonal Antibody at dilution of  1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of A-431 cells using RPL23A Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using RPL23A Polyclonal Antibody at dilution of  1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using RPL23A Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Overview

Circle
Product Name
RPL23A Polyclonal Antibody
Catalog Number
RD83020A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L23P family of ribosomal proteins. It is located in the cytoplasm. The protein may be one of the target molecules involved in mediating growth inhibition by interferon. In yeast, the corresponding protein binds to a specific site on the 26S rRNA. This gene is co-transcribed with the U42A, U42B, U101A, and U101B small nucleolar RNA genes, which are located in its third, first, second, and fourth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human RPL23A (NP_000975.2).
Gene ID
6147
Swissprot
P62750
Synonyms
RPL23AL23AMDA20
Calculated MW
17 kDa
Observed MW
20 kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IF 1:50-1:100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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