Ready-To-Use ELISA Kits

ELISA (enzyme-linked immunosorbent assay) is a common laboratory technique used to detect and quantify the amount of analyte in a given sample. 
The Ready-To-Use ELISA kit is an optimized version of our Traditional kit that offers pre-diluted detection reagents, a shorter experimental time, and enhanced stability for a 16-month shelf life. 

Browse Ready-To-Use ELISA Kit

• Expansive target catalogue - over 6500 different kits
• Straightforward procedure - easy-to-follow steps
• Pre-diluted detection reagents - one less prep step
• Shorter experimental time - done in 3.5 hours
• Enhanced stability - store at -20^oC for up to 16 months

• Pre-coated 96-well strip plate
• 2 Plate sealers for 96 wells 
• 2 Standards
• Standard Diluent 
• Wash Buffer (30× concentrate)
• Detection Solution A  
• Detection Solution B 
• TMB Substrate 
• Stop Solution 
• Instruction manual

Ready-To-Use ELISA Kit Procedure:

1. Prepare all reagents, samples and standards;
2. Add 100 µL standard or sample to each well. Incubate 90 minutes at 37°C;
3. Aspirate and add 100 µL Detection Solution A. Incubate 45 minutes at 37°C;
4. Aspirate and wash 3 times;
5. Add 100 µL Detection Solution B. Incubate 45 minutes at 37°C; 
6. Aspirate and wash 5 times;
7. Add 90 µL Substrate Solution. Incubate 15-25 minutes at 37°C;
8. Add 50 µL Stop Solution. Read at 450 nm immediately.