α-SMA Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD72843A
Western Blot analysis of 3T3, Hela cells using α-SMA Polyclonal Antibody at dilution of 1:1500.
Western Blot analysis of 3T3, Hela cells using α-SMA Polyclonal Antibody at dilution of 1:1500.
Immunohistochemistry of paraffin-embedded Rat lung tissue using α-SMA Polyclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded Rat lung tissue using α-SMA Polyclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of Mouse heart tissue using α-SMA Polyclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of Mouse heart tissue using α-SMA Polyclonal Antibody at dilution of 1:200.
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Overview

Circle
Product Name
α-SMA Polyclonal Antibody
Catalog Number
RD72843A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

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Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 (Actin, Alpha 2, Smooth Muscle, Aorta) is a Protein Coding gene. Diseases associated with ACTA2 include Multisystemic Smooth Muscle Dysfunction Syndrome and Moyamoya Disease 5. Among its related pathways are ICos-ICosL Pathway in T-Helper Cell and GPCR Pathway. GO annotations related to this gene include protein kinase binding. An important paralog of this gene is ACTG2. ACTA1 (Actin, Alpha 1, Skeletal Muscle) is a Protein Coding gene. Diseases associated with ACTA1 include Nemaline Myopathy 3, Autosomal Dominant Or Recessive and Myopathy, Congenital, With Fiber-Type Disproportion. Among its related pathways are ICos-ICosL Pathway in T-Helper Cell and GPCR Pathway. GO annotations related to this gene include structural constituent of cytoskeleton and myosin binding. An important paralog of this gene is ACTC1. ACTG2 (Actin, Gamma 2, Smooth Muscle, Enteric) is a Protein Coding gene. Diseases associated with ACTG2 include Visceral Myopathy and Chronic Intestinal Pseudoobstruction. Among its related pathways are ICos-ICosL Pathway in T-Helper Cell and GPCR Pathway. An important paralog of this gene is ACTA2.

Immunogen Information

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Immunogen
Synthesized peptide derived from α-SMA
Swissprot
P62736,P68133,P63267
Synonyms
a actinAAT6ACTAACTA2Actin alpha 2 smooth muscle aortaActin aortic smooth muscleActinaortic smooth muscleACTSAACTVSAlpha 2 actinAlpha actin 2Alpha cardiac actinalpha smaAlpha-actin-2Cell growth inhibiting gene 46 proteinCell growth-inhibiti
Calculated MW
42 kDa
Observed MW
42 kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p,IF,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-2000, IHC 1:50-300, IF 1:50-1:200, ELISA 1:10000-20000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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