Traditional ELISA Kits

ELISA (enzyme-linked immunosorbent assay) is a common laboratory technique used to detect and quantify the amount of analyte in a given sample. The Traditional ELISA kit is our foundational ELISA kit that has a conventional workflow at a reasonable price, while maintaining the same high level of quality and sensitivity.

Browse Traditional ELISA Kits

• Expansive target catalogue - over 6500 different kits
• Many different reactivities available -including hard to find species
• Straightforward procedure - easy-to-follow steps
• Affordable - prices to fit all budgets including large projects
• Complete - includes everything you need except your samples

• Pre-coated 96-well strip plate
• 2 Plate sealers for 96 wells 
• 2 Standards
• Diluent Buffer
• Wash Buffer (30× concentrate)
• Detection Reagent A  
• Detection Reagent B 
• TMB Substrate 
• Stop Solution 
• Instruction manual

Traditional ELISA Kit Procedure:

1. Prepare all reagents, samples and standards;
2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100 µL prepared Detection Reagent B. Incubate 1 hour at 37°C; 
6. Aspirate and wash 5 times;
7. Add 90 µL Substrate Solution. Incubate 15-25 minutes at 37°C;
8. Add 50 µL Stop Solution. Read at 450 nm immediately.