EASYStep Pro ELISA Kits

ELISA (enzyme-linked immunosorbent assay) is a common laboratory technique used to detect and quantify the amount of analyte in a given sample. 
EASYStep Pro ELISA kits are an optimized version of our EASYStep ELISA kits that feature an even shorter experimental time and only two incubation steps.

Browse EASYStep Pro ELISA Kits

• Even shorter experimental time - done in 1.5 hours!
• Very easy procedure - only 2 incubation steps
• Small sample volume - only 50uL needed
• Available for 62 popular targets
• Easy storage - store at 4^oC for up to 6 months

• Pre-coated 96-well micro ELISA plate 
• 2 Standards
• Diluent Buffer Pro
• Wash Buffer (25× concentrate) 
• Detection Reagent Pro (100x concentrate)
• Detection Reagent Diluent
• Substrate Reagent
• Stop Solution 
• 5 Plate sealers 
• Instruction manual

1. Add 50 µL standard or sample to each well, immediately add add 50 μL of Detection Reagent Pro working solution to each well. 
   Incubate for 60 minutes at 37°C.
2. Aspirate and wash the plate for 5 times. 
3. Add 90 µL Substrate Solution. Incubate for 15 minutes at 37°C. 
4. Add 50 µL Stop Solution. Read at 450 nm immediately.