(KD Validated) eIF4E Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90369A
Western blot analysis of extracts from wild type(WT) and eIF4E knockdown (KD) 293T(KD) cells, using eIF4E Polyclonal Antibody at 1:500 dilution
Immunohistochemistry of paraffin-embedded Rat testis using EIF4E Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Mouse liver using EIF4E Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Human liver using EIF4E Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
![Immunofluorescence analysis of HeLa cells using [KD Validated] eIF4E Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](/upload/ec/antibodies/RD90369A-IF01.jpg)
Immunofluorescence analysis of HeLa cells using [KD Validated] eIF4E Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
![Immunofluorescence analysis of HepG2 cells using [KD Validated] eIF4E Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](/upload/ec/antibodies/RD90369A-IF02.jpg)
Immunofluorescence analysis of HepG2 cells using [KD Validated] eIF4E Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
![Immunofluorescence analysis of NIH/3T3 cells using [KD Validated] eIF4E Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining](/upload/ec/antibodies/RD90369A-IF03.jpg)
Immunofluorescence analysis of NIH/3T3 cells using [KD Validated] eIF4E Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining
![Immunofluorescence analysis of PC-12 cells using [KD Validated] eIF4E Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](/upload/ec/antibodies/RD90369A-IF04.jpg)
Immunofluorescence analysis of PC-12 cells using [KD Validated] eIF4E Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Product Name
(KD Validated) eIF4E Polyclonal Antibody
Catalog Number
RD90369A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.
Immunogen Information
Immunogen
Recombinant fusion protein of human eIF4E
Gene ID
1977
Swissprot
P06730
Synonyms
AUTS19CBPEIF4E1EIF4EL1EIF4FeIF-4EEIF4E
Calculated MW
58kDa/59kDa/60kDa
Observed MW
65KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas