(KD Validated) NDUFS5 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD89416A
Western blot analysis of extracts from wild type(WT) and NDUFS5 knockdown (KD) 293T cells, using NDUFS5 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts from wild type(WT) and NDUFS5 knockdown (KD) 293T cells, using NDUFS5 Polyclonal Antibody at 1:1000 dilution.
Immunofluorescence analysis of L929 cells using NDUFS5 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using NDUFS5 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Overview

Circle
Product Name
(KD Validated) NDUFS5 Polyclonal Antibody
Catalog Number
RD89416A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
This gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. Alternative splicing results in multiple transcript variants and pseudogenes have been identified on chromosomes 1, 4 and 17.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human NDUFS5
Gene ID
4725
Swissprot
O43920
Synonyms
NDUFS5CI-15kCI15KNADH:ubiquinone oxidoreductase subunit S5
Calculated MW
12kDa
Observed MW
Refer to figures

Applications

Circle
Reactivity
Human,Mouse
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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