(KD Validated) PROM1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90331A
Western blot analysis of extracts of HT-29 cells using PROM1 Polyclonal Antibody at 1:400 dilution.
Western blot analysis of extracts from wild type(WT) and PROM1 knockdown (KD) HCT116 cells using PROM1 Polyclonal Antibody at 1:400 dilution.
Immunohistochemistry of paraffin-embedded human colon carcinoma using CD133 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat kidney using CD133 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat lung using CD133 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using CD133 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HT-29 cells using PROM1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HT-29 cells using 3ug CD133 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using CD133 Polyclonal Antibody at a dilution of 1:1000.
Product Name
(KD Validated) PROM1 Polyclonal Antibody
Catalog Number
RD90331A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene.
Immunogen Information
Immunogen
Recombinant fusion protein of human PROM1
Gene ID
8842
Swissprot
O43490
Synonyms
PROM1AC133CD133CORD12MCDR2MSTP061PROML1RP41STGD4prominin-1
Calculated MW
38kDa/47kDa
Observed MW
62KDa
Applications
Reactivity
Human,Rat
Tested Applications
WB,IHC,IF,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:100,IF 1:50-1:200,IP 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas