For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89476A
Western blot analysis of extracts from wild type(WT) and SFRP2 knockdown (KD) HeLa cells, using SFRP2 Polyclonal Antibody at 1:1000 dilution.
Immunofluorescence analysis of A431 cells using [KD Validated] SFRP2 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using [KD Validated] SFRP2 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using [KD Validated] SFRP2 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer.
Immunogen Information
Immunogen
A synthetic peptide of human SFRP2
Gene ID
6423
Swissprot
Q96HF1
Synonyms
SFRP2FRP-2SARP1SDF-5
Calculated MW
114kDa
Observed MW
150KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.