(KO Validated) Beclin 1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90330A
Western blot analysis of extracts of various cell lines using Beclin 1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human stomach using Beclin 1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse kidney using Beclin 1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
![Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Beclin 1 Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.](/upload/ec/antibodies/RD90330A-IF01.jpg)
Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Beclin 1 Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
![Immunofluorescence analysis of PC-12 cells using [KO Validated] Beclin 1 Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.](/upload/ec/antibodies/RD90330A-IF02.jpg)
Immunofluorescence analysis of PC-12 cells using [KO Validated] Beclin 1 Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Product Name
(KO Validated) Beclin 1 Polyclonal Antibody
Catalog Number
RD90330A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.
Immunogen Information
Immunogen
Recombinant fusion protein of human Beclin 1
Gene ID
8678
Swissprot
Q14457
Synonyms
BECN1ATG6VPS30beclin1beclin-1
Calculated MW
92kDa/93kDa/94kDa/96kDa/97kDa
Observed MW
120KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:100,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.