(KO Validated) CDK2 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90187A
Western blot analysis of extracts of various cell lines using CDK2 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts from normal (control) and CDK2 knockout (KO) 293T cells using CDK2 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of HeLa cells using CDK2 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human colon carcinoma using CDK2 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
![Immunofluorescence analysis of HeLa cells using [KO Validated] CDK2 Polyclonal Antibody at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.](/upload/ec/antibodies/RD90187A-IF01.jpg)
Immunofluorescence analysis of HeLa cells using [KO Validated] CDK2 Polyclonal Antibody at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.
![Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] CDK2 Polyclonal Antibody at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining](/upload/ec/antibodies/RD90187A-IF02.jpg)
Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] CDK2 Polyclonal Antibody at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining
![Immunofluorescence analysis of PC-12 cells using [KO Validated] CDK2 Polyclonal Antibody at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.](/upload/ec/antibodies/RD90187A-IF03.jpg)
Immunofluorescence analysis of PC-12 cells using [KO Validated] CDK2 Polyclonal Antibody at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.
![Immunofluorescence analysis of U2OS cells using [KO Validated] CDK2 Polyclonal Antibody at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.](/upload/ec/antibodies/RD90187A-IF04.jpg)
Immunofluorescence analysis of U2OS cells using [KO Validated] CDK2 Polyclonal Antibody at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.
Product Name
(KO Validated) CDK2 Polyclonal Antibody
Catalog Number
RD90187A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants.
Immunogen Information
Immunogen
A synthetic peptide of human CDK2
Gene ID
1017
Swissprot
P24941
Synonyms
CDK2CDKN2p33(CDK2)
Calculated MW
119kDa/128kDa/134kDa
Observed MW
180kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas