(KO Validated) CDK4 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90288A
Western blot analysis of extracts of various cell lines using CDK4 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of 293T cells using CDK4 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts from wild type(WT) and CDK4 knockout (KO) HeLa cells using CDK4 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human colon using CDK4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH/3T3 using CDK4 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug CDK4 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using CDK4 Polyclonal Antibody at a dilution of 1:1000.
Variation
Quantity
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Overview
Product Name
(KO Validated) CDK4 Polyclonal Antibody
Catalog Number
RD90288A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.
Immunogen Information
Immunogen
A synthetic peptide of human CDK4
Gene ID
1019
Swissprot
P11802
Synonyms
CDK4CMM3PSK-J3cyclin-dependent kinase 4
Calculated MW
18kDa
Observed MW
Refer to figures
Applications
Reactivity
Human,Mouse
Tested Applications
WB,IHC,IP,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:100,IP 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas