(KO Validated) CDKN2A / p16INK4a Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD86748A
Western blot analysis of extracts of HeLa cells using CDKN2A/p16INK4a Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of HeLa cells using CDKN2A/p16INK4a Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts from normal (control) and CDKN2A/p16INK4a knockout (KO) 293T cells using CDKN2A/p16INK4a Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts from normal (control) and CDKN2A/p16INK4a knockout (KO) 293T cells using CDKN2A/p16INK4a Polyclonal Antibody at 1:1000 dilution.
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Overview

Circle
Product Name
(KO Validated) CDKN2A / p16INK4a Polyclonal Antibody
Catalog Number
RD86748A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.

Immunogen Information

Circle
Immunogen
A synthetic peptide of human CDKN2A / p16INK4a
Gene ID
1029
Swissprot
P42771,Q8N726
Synonyms
CDKN2AARFCDK4ICDKN2CMM2INK4INK4AMLMMTS-1MTS1P14P14ARFP16P16-INK4AP16INK4P16INK4AP19P19ARFTP16
Calculated MW
25kDa/35kDa/36kDa
Observed MW
43kDa

Applications

Circle
Reactivity
Human
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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