(KO Validated) EGFR Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD82507A
Western blot analysis of extracts from normal (control) and EGFR knockout (KO) HeLa cells using EGFR Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using EGFR Polyclonal Antibody at dilution of 1:1000.
Product Name
(KO Validated) EGFR Polyclonal Antibody
Catalog Number
RD82507A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.
Immunogen Information
Immunogen
A synthetic peptide of human EGFR (NP_005219.2).
Gene ID
1956
Swissprot
P00533
Synonyms
EGFRERBBERBB1HER1NISBD2PIG61mENA
Calculated MW
44 kDa/69 kDa/77 kDa/134 kDa
Observed MW
175 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas