Western blot analysis of extracts from normal (control) and Glutathione Synthetase (Glutathione Synthetase (GSS)) knockout (KO) 293T cells using Glutathione Synthetase (Glutathione Synthetase (GSS)) Polyclonal Antibody at 1:1000 dilution.
Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Glutathione Synthetase (GSS) Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using [KO Validated] Glutathione Synthetase (GSS) Polyclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using [KO Validated] Glutathione Synthetase (GSS) Polyclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.
Immunogen Information
Immunogen
Recombinant fusion protein of human GSS
Gene ID
2937
Swissprot
P48637
Synonyms
GSSGSHSHEL-S-64pHEL-S-88n
Calculated MW
32kDa/33kDa/35kDa
Observed MW
37kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.