(KO Validated) GSS Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86785A
Western blot analysis of extracts from normal (control) and Glutathione Synthetase (Glutathione Synthetase (GSS)) knockout (KO) 293T cells using Glutathione Synthetase (Glutathione Synthetase (GSS)) Polyclonal Antibody at 1:1000 dilution.
![Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Glutathione Synthetase (GSS) Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](/upload/ec/antibodies/RD86785A-IF01.jpg)
Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Glutathione Synthetase (GSS) Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
![Immunofluorescence analysis of PC-12 cells using [KO Validated] Glutathione Synthetase (GSS) Polyclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](/upload/ec/antibodies/RD86785A-IF02.jpg)
Immunofluorescence analysis of PC-12 cells using [KO Validated] Glutathione Synthetase (GSS) Polyclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
![Immunofluorescence analysis of U2OS cells using [KO Validated] Glutathione Synthetase (GSS) Polyclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](/upload/ec/antibodies/RD86785A-IF03.jpg)
Immunofluorescence analysis of U2OS cells using [KO Validated] Glutathione Synthetase (GSS) Polyclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
(KO Validated) GSS Polyclonal Antibody
Catalog Number
RD86785A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.
Immunogen Information
Immunogen
Recombinant fusion protein of human GSS
Gene ID
2937
Swissprot
P48637
Synonyms
GSSGSHSHEL-S-64pHEL-S-88n
Calculated MW
32kDa/33kDa/35kDa
Observed MW
37kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas