(KO Validated) HIF1AN Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86836A
Western blot analysis of extracts of various cell lines using HIF1AN/FIH1 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts from normal (control) and HIF1AN/FIH1 antibody knockout (KO) 293T cells, using HIF1AN/FIH1 antibody at 1:1000 dilution.
Immunofluorescence analysis of L929 cells using HIF1AN/FIH1 Polyclonal antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
(KO Validated) HIF1AN Polyclonal Antibody
Catalog Number
RD86836A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD. Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.
Immunogen Information
Immunogen
Recombinant fusion protein of human HIF1AN
Gene ID
55662
Swissprot
Q9NWT6
Synonyms
HIF1ANFIH1
Calculated MW
14kDa
Observed MW
14kDa/16kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas