(KO Validated) HMGB1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86821A
Western blot analysis of various lysates using HMGB1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat brain using HMGB1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human breast cancer using HMGB1 Polyclonal antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse heart using HMGB1 Polyclonal antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Name
(KO Validated) HMGB1 Polyclonal Antibody
Catalog Number
RD86821A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.
Immunogen Information
Immunogen
A synthetic peptide of human HMGB1
Gene ID
3146
Swissprot
P09429
Synonyms
HMG-1HMG1HMG3SBP-1HMGB1
Calculated MW
23-55kDa
Observed MW
28kDa/55kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas