(KO Validated) MSH2 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Western blot analysis of A-549 using MSH2 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts from wild type(WT) and MSH2 knockout (KO) HeLa cells using MSH2 Polyclonal Antibody at 1:1000 dilution.
Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug MSH2 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using MSH2 Polyclonal Antibody at a dilution of 1:3000.
Product Name
(KO Validated) MSH2 Polyclonal Antibody
Catalog Number
RD89412A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene.
Immunogen Information
Immunogen
Recombinant fusion protein of human MSH2
Gene ID
4436
Swissprot
P43246
Synonyms
MSH2COCA1FCC1HNPCCHNPCC1LCFS2mutS homolog 2
Calculated MW
35kDa/48kDa/51kDa
Observed MW
Refer to figures
Applications
Reactivity
Human,Rat
Tested Applications
WB,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IP 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas