(KO Validated) N-Cadherin Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90285A
Western blot analysis of extracts of various cell lines using N-Cadherin Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of HeLa cells using N-Cadherin Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of 293T cells using N-Cadherin Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts from wild type(WT) and N-Cadherin knockout (KO) HeLa cells using N-Cadherin Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded Human colon carcinoma using N-Cadherin Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Mouse liver using N-Cadherin Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 600ug extracts of Mouse brain using 3ug N-Cadherin Polyclonal Antibody. Western blot was performed from the immunoprecipitate using N-Cadherin Polyclonal Antibody at a dilution of 1:1000.
Variation
Quantity
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Overview
Product Name
(KO Validated) N-Cadherin Polyclonal Antibody
Catalog Number
RD90285A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone.
Immunogen Information
Immunogen
A synthetic peptide of human N-Cadherin
Gene ID
1000
Swissprot
P19022
Synonyms
CDH2CD325CDHNCDw325NCADcadherin-2
Calculated MW
60kDa/78kDa/79kDa/80kDa/81kDa/82kDa/83kDa
Observed MW
78-82KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IP 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas