(KO Validated) NDUFB4 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD81613A
Western blot analysis of extracts of various cell lines using NDUFB4 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using NDUFB4 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts from normal (control) and NDUFB4 knockout (KO) HeLa cells using NDUFB4 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts from normal (control) and NDUFB4 knockout (KO) HeLa cells using NDUFB4 Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of L929 cells using NDUFB4 Polyclonal Antibody at dilution of  1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using NDUFB4 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using NDUFB4 Polyclonal Antibody at dilution of  1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using NDUFB4 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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For more information on our kits, please contact us at info@reddotbiotech.com

Overview

Circle
Product Name
(KO Validated) NDUFB4 Polyclonal Antibody
Catalog Number
RD81613A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
This gene encodes a non-catalytic subunit of the multisubunit NADH:ubiquinone oxidoreductase, the first enzyme complex in the mitochondrial electron transport chain (complex I). Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to ubiquinone.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human NDUFB4 (NP_001161803.1).
Gene ID
4710
Swissprot
O95168
Synonyms
NDUFB4B15CI-B15
Calculated MW
14 kDa/15 kDa
Observed MW
15 kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IF 1:50-1:100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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