(KO Validated) NSDHL Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD88691A
Western blot analysis of extracts from wild type(WT) and NSDHL knockout (KO) 293T(KO) cells, using NSDHL Polyclonal Antibody at 1:1000 dilution.
![Immunohistochemistry of paraffin-embedded Human liver cancer using [KO Validated] NSDHL Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](/upload/ec/antibodies/RD88691A-IHC01.jpg)
Immunohistochemistry of paraffin-embedded Human liver cancer using [KO Validated] NSDHL Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
![Immunohistochemistry of paraffin-embedded Mouse heart using [KO Validated] NSDHL Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](/upload/ec/antibodies/RD88691A-IHC02.jpg)
Immunohistochemistry of paraffin-embedded Mouse heart using [KO Validated] NSDHL Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using NSDHL Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using NSDHL Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Variation
Quantity
For more information on our kits, please contact us at info@reddotbiotech.com
Overview
Product Name
(KO Validated) NSDHL Polyclonal Antibody
Catalog Number
RD88691A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene is localized in the endoplasmic reticulum and is involved in cholesterol biosynthesis. Mutations in this gene are associated with CHILD syndrome, which is a X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, and typically lethal in males. Alternatively spliced transcript variants with differing 5' UTR have been found for this gene.
Immunogen Information
Immunogen
Recombinant fusion protein of human NSDHL
Gene ID
50814
Swissprot
Q15738
Synonyms
NSDHLH105E3SDR31E1XAP104
Calculated MW
17kDa
Observed MW
14-17KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.