(KO Validated) NUCB1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD83003A
Western blot analysis of extracts of various cell lines using NUCB1 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts from normal (control) and NUCB1 knockout (KO) 293T cells using NUCB1 Polyclonal Antibody at dilution of 1:3000.
Immunofluorescence analysis of L929 cells using NUCB1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using NUCB1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Variation
Quantity
For more information on our kits, please contact us at info@reddotbiotech.com
Overview
Product Name
(KO Validated) NUCB1 Polyclonal Antibody
Catalog Number
RD83003A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a member of a small calcium-binding EF-hand protein family. The encoded protein is thought to have a key role in Golgi calcium homeostasis and Ca(2+)-regulated signal transduction events.
Immunogen Information
Immunogen
Recombinant fusion protein of human NUCB1 (NP_006175.2).
Gene ID
4924
Swissprot
Q02818
Synonyms
NUCB1CALNUCNUC
Calculated MW
53 kDa
Observed MW
60 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IF 1:50-1:100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas