(KO Validated) PDXK Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89454A
Western blot analysis of extracts from normal (control) and PDXK knockout (KO) 293T cells, using PDXK Polyclonal Antibody at 1:1000 dilution.
Immunofluorescence analysis of NIH-3T3 using PDXK Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa using PDXK Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa using PDXK Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
(KO Validated) PDXK Polyclonal Antibody
Catalog Number
RD89454A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
Immunogen Information
Immunogen
Recombinant fusion protein of human PDXK
Gene ID
8566
Swissprot
O00764
Synonyms
PDXKC21orf124C21orf97HEL-S-1aPKHPNKPRED79pyridoxal kinase
Calculated MW
37kDa
Observed MW
Refer to figures
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.