(KO Validated) PEPCK/PCK2 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89419A
Western blot analysis of various lysates using PEPCK/PCK2 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts from wild type(WT) and PEPCK/PCK2 knockout (KO) 293T cells using PEPCK/PCK2 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded mouse kidney using PEPCK/PEPCK/PCK2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat kidney using PEPCK/PEPCK/PCK2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH-3T3 cells using PEPCK/PEPCK/PCK2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using PEPCK/PEPCK/PCK2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
(KO Validated) PEPCK/PCK2 Polyclonal Antibody
Catalog Number
RD89419A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described.
Immunogen Information
Immunogen
Recombinant fusion protein of human PEPCK/PCK2
Gene ID
5106
Swissprot
Q16822
Synonyms
PCK2PEPCKPEPCK-MPEPCK2phosphoenolpyruvate carboxykinase 2mitochondrial
Calculated MW
32kDa/40kDa/44kDa
Observed MW
41KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200,IP 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas