(KO Validated) PHD2/EGLN1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87929A
Western blot analysis of extracts from normal (control) and PHD2/EGLN1 knockout (KO) 293T cells, using PHD2/EGLN1 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using PHD2/EGLN1 Polyclonal Antibody at 1:1000 dilution.
![Immunohistochemistry of paraffin-embedded mouse brain using [KO Validated] PHD2/EGLN1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](/upload/ec/antibodies/RD87929A-IHC01.jpg)
Immunohistochemistry of paraffin-embedded mouse brain using [KO Validated] PHD2/EGLN1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
![Immunohistochemistry of paraffin-embedded rat brain using [KO Validated] PHD2/EGLN1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](/upload/ec/antibodies/RD87929A-IHC02.jpg)
Immunohistochemistry of paraffin-embedded rat brain using [KO Validated] PHD2/EGLN1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
![Immunohistochemistry of paraffin-embedded rat heart using [KO Validated] PHD2/EGLN1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](/upload/ec/antibodies/RD87929A-IHC03.jpg)
Immunohistochemistry of paraffin-embedded rat heart using [KO Validated] PHD2/EGLN1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
![Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] PHD2/EGLN1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](/upload/ec/antibodies/RD87929A-IF01.jpg)
Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] PHD2/EGLN1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Product Name
(KO Validated) PHD2/EGLN1 Polyclonal Antibody
Catalog Number
RD87929A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3).
Immunogen Information
Immunogen
A synthetic peptide of human PHD2/EGLN1
Gene ID
54583
Swissprot
Q9GZT9
Synonyms
EGLN1C1orf12ECYT3HALAHHIF-PH2HIFPH2HPH-2HPH2PHD2SM20ZMYND6
Calculated MW
39kDa
Observed MW
38kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.