(KO Validated) POR Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD84930A
Western blot analysis of extracts from normal (control) and POR knockout (KO) HeLa cells using POR Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using POR Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human esophagus using POR Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using POR Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using POR Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using POR Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Product Name
(KO Validated) POR Polyclonal Antibody
Catalog Number
RD84930A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome.
Immunogen Information
Immunogen
Recombinant fusion protein of human POR (NP_000932.3).
Gene ID
5447
Swissprot
P16435
Synonyms
PORCPRCYPORP450R
Calculated MW
76 kDa
Observed MW
77 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:100 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas