(KO Validated) SEC61B Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD266577A
Western blot analysis of extracts of various cell lines using SEC61B Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts from normal (control) and SEC61B knockout (KO) 293T cells using SEC61B Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of L929 cells using SEC61B Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Product Name
(KO Validated) SEC61B Polyclonal Antibody
Catalog Number
RD266577A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript.
Immunogen Information
Immunogen
A synthetic peptide of human SEC61B (NP_006799.1).
Gene ID
10952
Swissprot
P60468
Synonyms
SEC61B
Calculated MW
9 kDa
Observed MW
12 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.