(KO Validated) SQSTM1/p62 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90332A
Western blot analysis of extracts of various cell lines using SQSTM1/p62 Polyclonal Antibody at 1:4000 dilution.
Western blot analysis of extracts from wild type(WT) and SQSTM1/p62 knockout (KO) 293T cells using SQSTM1/p62 Polyclonal Antibody at 1:4000 dilution.
Immunohistochemistry of paraffin-embedded Human lung adenocarcinoma using SQSTM1/p62 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Human extranodal NK-T cell lymphoma using SQSTM1/p62 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining
Immunohistochemistry of paraffin-embedded Mouse kidney using SQSTM1/p62 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Rat spleen using SQSTM1/p62 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH/3T3 cells using SQSTM1/p62 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using SQSTM1/p62 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Product Name
(KO Validated) SQSTM1/p62 Polyclonal Antibody
Catalog Number
RD90332A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
Immunogen Information
Immunogen
Recombinant fusion protein of human SQSTM1/p62
Gene ID
8878
Swissprot
Q13501
Synonyms
SQSTM1A170DMRVFTDALS3NADGPOSILPDB3ZIP3p60p62p62Bsequestosome-1
Calculated MW
25kDa/33kDa/39kDa
Observed MW
67KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.