(KO Validated) SUMO1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89443A
Western blot analysis of extracts from wild type(WT) and SUMO1 knockout (KO) 293T cells, using SUMO1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human lung cancer using SUMO1 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat brain using SUMO1 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse testis using SUMO1 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using SUMO1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using SUMO1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using SUMO1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Product Name
(KO Validated) SUMO1 Polyclonal Antibody
Catalog Number
RD89443A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized.
Immunogen Information
Immunogen
A synthetic peptide of human SUMO1
Gene ID
7341
Swissprot
P63165
Synonyms
SUMO1DAP1GMP1OFC10PIC1SENP2SMT3SMT3CSMT3H3UBL1small ubiquitin-like modifier 1
Calculated MW
35kDa/103kDa/108kDa
Observed MW
Refer to figures
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:100,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas